BMRB Entry 6129

Name: Solution structure and backbone dynamics of the Cu(I) and apo-forms of the second metal-binding domain of the Menkes protein ATP7A
Published: 2004-05-15

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PDB ID: 1s6u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6129
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure and backbone dynamics of the Cu(I) and apo-forms of the second metal-binding domain of the Menkes protein ATP7A

Deposition date:

2004-03-03

Original release date:

2004-05-15

Authors:

Banci, L.; Bertini, I.; Del Conte, R.; D'Onofrio, M.; Rosato, A.

Citation:

Citation: Banci, L.; Bertini, I.; Del Conte, R.; D'Onofrio, M.; Rosato, A.. "Solution structure and backbone dynamics of the Cu(I) and apo forms of the second metal-binding domain of the Menkes protein ATP7A" Biochemistry 43, 3396-3403 (2004).
PubMed: 15035611

Assembly members:

Assembly members:
ATPase, polymer, 76 residues, 63.546 Da.
CU, non-polymer, 63.546 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ATPase: GEVVLKMKVEGMTCHSCTST IEGKIGKLQGVQRIKVSLDN QEATIVYQPHLISVEEMKKQ IEAMGFPAFVKKIEGR

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	248
15N chemical shifts	78
1H chemical shifts	529

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Copper-transporting ATPase 1	1
2	COPPER (I) ION	2

Entities:

Entity 1, Copper-transporting ATPase 1 76 residues - 63.546 Da.

1

GLY

GLU

VAL

LEU

LYS

MET

LYS

VAL

GLU

2

GLY

MET

THR

CYS

HIS

SER

CYS

THR

SER

THR

3

ILE

GLU

GLY

LYS

ILE

GLY

LYS

LEU

GLN

GLY

4

VAL

GLN

ARG

ILE

LYS

VAL

SER

LEU

ASP

ASN

5

GLN

GLU

ALA

THR

ILE

VAL

TYR

GLN

PRO

HIS

6

LEU

ILE

SER

VAL

GLU

MET

LYS

GLN

7

ILE

GLU

ALA

MET

GLY

PHE

PRO

ALA

PHE

VAL

8

LYS

ILE

GLU

GLY

ARG

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1

CU

Samples:

sample_1: ATPase 1 mM; COPPER (I) ION 1 mM; phosphate 100 mM; H2O 90%; D2O 10%

sample_2: ATPase, [U-15N], 1 mM; COPPER (I) ION 1 mM; phosphate 100 mM; H2O 90%; D2O 10%

sample_3: ATPase, [U-13C; U-15N], 1 mM; COPPER (I) ION 1 mM; phosphate 100 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
[1H-1H]-NOESY	not available	not available	sample_cond_1
13C-NOESY-HSQC	not available	not available	sample_cond_1
15N-NOESY-HSQC	not available	not available	sample_cond_1
HNHA	not available	not available	sample_cond_1

Software:

DYANA v1.5 - refinement, structure solution

NMR spectrometers:

unknown unknown 0 MHz