BMRB Entry 5470

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PDB ID: 1iy6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5470
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Inhibitory Specificity Change of Ovomucoid Third Domain of Silver Pheasant upon Introduction of an Engineered Cys14-Cys39 Bond

Deposition date:

2002-07-22

Original release date:

2003-03-06

Authors:

Hemmi, Hikaru; Kumazaki, Takashi; Yamazaki, Toshimasa; Kojima, Shuichi; Yoshida, Takuya; Kyogoku, Yoshimasa; Katsu, Masataka; Shinohara, Fumikazu; Yokosawa, Hideyoshi; Miura, Kin-ichiro; Kobayashi, Yuji

Citation:

Citation: Hemmi, Hikaru; Kumazaki, Takashi; Yamazaki, Toshimasa; Kojima, Shuichi; Yoshida, Takuya; Kyogoku, Yoshimasa; Katsu, Masataka; Shinohara, Fumikazu; Yokosawa, Hideyoshi; Miura, Kin-ichiro; Kobayashi, Yuji. "Inhibitory Specificity Change of Ovomucoid Third Domain of Silver Pheasant upon Introduction of an Engineered Cys14-Cys39 Bond" Biochemistry 42, 2524-2534 (2003).

Assembly members:

Assembly members:
ovomucoid third domain from silver pheasant, polymer, 54 residues, Formula weight is not available

Natural source:

Natural source: Common Name: silver pheasant Taxonomy ID: 9046 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lophura nycthemera

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ovomucoid third domain from silver pheasant: AVSVDCSEYPKCACTMEYRP LCGSDNKTYGNKCNFCCAVV ESNGTLTLSHFGKC

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	341

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p14c/n39c monomer	1

Entities:

Entity 1, p14c/n39c monomer 54 residues - Formula weight is not available

1

ALA

VAL

SER

VAL

ASP

CYS

SER

GLU

TYR

PRO

2

LYS

CYS

ALA

CYS

THR

MET

GLU

TYR

ARG

PRO

3

LEU

CYS

GLY

SER

ASP

ASN

LYS

THR

TYR

GLY

4

ASN

LYS

CYS

ASN

PHE

CYS

ALA

VAL

5

GLU

SER

ASN

GLY

THR

LEU

THR

LEU

SER

HIS

6

PHE

GLY

LYS

CYS

Samples:

sample_1: ovomucoid third domain from silver pheasant 4.0 mM

Ex-cond_1: pH: 4.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-1H NOESY	not available	not available	not available
1H-1H TOCSY	not available	not available	not available
1H-1H DQF-COSY	not available	not available	not available

Software:

NMRPipe - processing

PIPP - assignment, peak picking

NMR spectrometers:

Bruker AMX 500 MHz
Bruker DRX 600 MHz