BMRB Entry 5469
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5469
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Hemmi, Hikaru; Kumazaki, Takashi; Yamazaki, Toshimasa; Kojima, Shuichi; Yoshida, Takuya; Kyogoku, Yoshimasa; Katsu, Masataka; Shinohara, Fumikazu; Yokosawa, Hideyoshi; Miura, Kin-ichiro; Kobayashi, Yuji. "Inhibitory Specificity Change of Ovomucoid Third Domain of Silver Pheasant upon
Introduction of an Engineered Cys14-Cys39 Bond" Biochemistry 42, 2524-2534 (2003).
Assembly members:
ovomucoid third domain from silver pheasant, polymer, 54 residues, Formula weight is not available
Natural source: Common Name: silver pheasant Taxonomy ID: 9046 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lophura nycthemera
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b
Entity Sequences (FASTA):
ovomucoid third domain from silver pheasant: AVSVDCSEYPKPACTMEYRP
LCGSDNKTYGNKCNFCNAVV
ESNGTLTLSHFGKC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 343 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | omsvp3 monomer | 1 |
Entities:
Entity 1, omsvp3 monomer 54 residues - Formula weight is not available
| 1 | ALA | VAL | SER | VAL | ASP | CYS | SER | GLU | TYR | PRO | ||||
| 2 | LYS | PRO | ALA | CYS | THR | MET | GLU | TYR | ARG | PRO | ||||
| 3 | LEU | CYS | GLY | SER | ASP | ASN | LYS | THR | TYR | GLY | ||||
| 4 | ASN | LYS | CYS | ASN | PHE | CYS | ASN | ALA | VAL | VAL | ||||
| 5 | GLU | SER | ASN | GLY | THR | LEU | THR | LEU | SER | HIS | ||||
| 6 | PHE | GLY | LYS | CYS |
Samples:
sample_1: ovomucoid third domain from silver pheasant 4.0 mM
Ex-cond_1: pH: 4.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H NOESY | not available | not available | not available |
| 1H-1H TOCSY | not available | not available | not available |
| 1H-1H DQF-COSY | not available | not available | not available |
Software:
NMRPipe - processing
NMRPIPP - assignment, peak picking
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DRX 600 MHz