BMRB Entry 4281

Name: Backbone and Side Chain 1H, 13C, and 15N Chemical Shift Assignments for AbrBN
Published: 2005-08-05

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PDB ID: 1z0r
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4281
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and Side Chain 1H, 13C, and 15N Chemical Shift Assignments for AbrBN

Deposition date:

1998-12-10

Original release date:

2005-08-05

Authors:

Bobay, Benjamin; Andreeva, Antonina; Mueller, Geoffrey; Cavanagh, John; Murzin, Alexey

Citation:

Citation: Bobay, Benjamin; Andreeva, Antonina; Mueller, Geoffrey; Cavanagh, John; Murzin, Alexey. "Revised structure of the AbrB N-terminal domain unifies a diverse superfamily of putative DNA-binding proteins" .

Assembly members:

Assembly members:
AbrB, polymer, 53 residues, 6100 Da.

Natural source:

Natural source: Common Name: B. subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AbrB: MKSTGIVRKVDELGRVVIPI ELRRTLGIAEKDALEIYVDD EKIILKKYKPNMT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
1H chemical shifts	403
13C chemical shifts	238
15N chemical shifts	54

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AbrBN subunit 1	1
2	AbrBN subunit 2	1

Entities:

Entity 1, AbrBN subunit 1 53 residues - 6100 Da.

1

MET

LYS

SER

THR

GLY

ILE

VAL

ARG

LYS

VAL

2

ASP

GLU

LEU

GLY

ARG

VAL

ILE

PRO

ILE

3

GLU

LEU

ARG

THR

LEU

GLY

ILE

ALA

GLU

4

LYS

ASP

ALA

LEU

GLU

ILE

TYR

VAL

ASP

5

GLU

LYS

ILE

LEU

LYS

TYR

LYS

PRO

6

ASN

MET

THR

Samples:

sample_one: AbrB, [U-99% 13C; U-99% 15N], 1.5 – 2.5 mM; KCl 15 mM; K-PO 10 mM; DTT 1 mM; NaN3 0.02%; D20 5%; H2O 95%

sample_two: AbrB, [U-99% 13C; U-99% 15N], 1.5 – 2.5 mM; KCl 15 mM; K-PO 10 mM; DTT 1 mM; NaN3 0.02%; D20 5%; H2O 95%

standard_h2o_conditions: pH: 5.8; temperature: 305 K

standard_d2o_conditions: pH*: 5.8; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY (120mS and 150mS mixing time)	not available	not available	not available
1H-13C COESY (120mS and 120mS folded mixing time)	not available	not available	not available
3-D HNCA	not available	not available	not available
3-D HN(CO)CA	not available	not available	not available
3-D HNCO	not available	not available	not available
3-D HN(CA)CO	not available	not available	not available
3-D CBCA(CO)NH	not available	not available	not available
3-D HNCACB	not available	not available	not available
D2O exchange via 2-D 1H-15N HSQC	not available	not available	not available
CC-TOCSY-(CO)NH	not available	not available	not available
H(CC-TOCSY-CO)NH	not available	not available	not available
HCCH-TOCSY	not available	not available	not available

Software:

ARIA v1.2 -

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	17650
PDB	1YFB 1YSF 1Z0R 2K1N 2RO4
DBJ	BAA05272 BAI83483 BAL15827 BAM48968 BAM56238
EMBL	CAA31307 CAA43955 CAB11813 CBI41163 CCF03572
GB	AAA22195 AAP07140 AAP24090 AAS38971 AAT29114
PRF	1715209A
REF	NP_387918 NP_829939 NP_842604 WP_000843034 WP_000843035
SP	P08874
AlphaFold	P08874