BMRB Entry 4232

Name: N-Domain of Troponin C from Chicken Skeletal Muscle.
Published: 1999-05-25

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PDB ID: 1zac
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4232
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

N-Domain of Troponin C from Chicken Skeletal Muscle.

Deposition date:

1998-09-27

Original release date:

1999-05-25

Authors:

Tsuda, Sakae; Miura, A.; Gagne, S.; Spyracopoulos, L.; Sykes, Brian

Citation:

Citation: Tsuda, Sakae; Miura, A.; Gagne, S.; Spyracopoulos, L.; Sykes, Brian. "Low Temperature-Induced Structural Changes in the Apo Regulatory Domain of Skeletal Muscle Troponin C" Biochemistry 38, 5693-5700 (1999).

Assembly members:

Assembly members:
apoTroponin-C, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apoTroponin-C: ASMTDQQAEARAFLSEEMIA EFKAAFDMFDADGGGDISTK ELGTVMRMLGQNPTKEELDA IIEEVDEDGSGTIDFEEFLV MMVRQMKEDA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one
- assigned_chemical_shifts_two
coupling_constants
- assigned_coupling_constants_one

Data type	Count
13C chemical shifts	259
15N chemical shifts	184
1H chemical shifts	770
coupling constants	81

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	apoNTnC	1

Entities:

Entity 1, apoNTnC 90 residues - Formula weight is not available

1	ALA	SER	MET	THR	ASP	GLN	GLN	ALA	GLU	ALA
2	ARG	ALA	PHE	LEU	SER	GLU	GLU	MET	ILE	ALA
3	GLU	PHE	LYS	ALA	ALA	PHE	ASP	MET	PHE	ASP
4	ALA	ASP	GLY	GLY	GLY	ASP	ILE	SER	THR	LYS
5	GLU	LEU	GLY	THR	VAL	MET	ARG	MET	LEU	GLY
6	GLN	ASN	PRO	THR	LYS	GLU	GLU	LEU	ASP	ALA
7	ILE	ILE	GLU	GLU	VAL	ASP	GLU	ASP	GLY	SER
8	GLY	THR	ILE	ASP	PHE	GLU	GLU	PHE	LEU	VAL
9	MET	MET	VAL	ARG	GLN	MET	LYS	GLU	ASP	ALA

Samples:

sample_one: apoTroponin-C, [U-13C; U-15N], 1.5 mM; KCl 50 mM

sample_conditions_one: pH: 6.7; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
CBCACONNH	sample_one	not available	sample_conditions_one
15N-NOESY	sample_one	not available	sample_conditions_one
HNHA	sample_one	not available	sample_conditions_one

Software:

No software information available

NMR spectrometers:

Varian UNITY 500 MHz
Varian UNITY 600 MHz

BMRB	16414 4401
PDB	1AVS 1BLQ 1NPQ 1SKT 1SMG 1TNP 1TNQ 1TRF 1ZAC
GB	EMP28292 KFU86923
REF	XP_007067817 XP_009321443 XP_009582608 XP_009693659 XP_009992324

1	ALA	SER	MET	THR	ASP	GLN	GLN	ALA	GLU	ALA
2	ARG	ALA	PHE	LEU	SER	GLU	GLU	MET	ILE	ALA
3	GLU	PHE	LYS	ALA	ALA	PHE	ASP	MET	PHE	ASP
4	ALA	ASP	GLY	GLY	GLY	ASP	ILE	SER	THR	LYS
5	GLU	LEU	GLY	THR	VAL	MET	ARG	MET	LEU	GLY
6	GLN	ASN	PRO	THR	LYS	GLU	GLU	LEU	ASP	ALA
7	ILE	ILE	GLU	GLU	VAL	ASP	GLU	ASP	GLY	SER
8	GLY	THR	ILE	ASP	PHE	GLU	GLU	PHE	LEU	VAL
9	MET	MET	VAL	ARG	GLN	MET	LYS	GLU	ASP	ALA

1	ALA	SER	MET	THR	ASP	GLN	GLN	ALA	GLU	ALA
2	ARG	ALA	PHE	LEU	SER	GLU	GLU	MET	ILE	ALA
3	GLU	PHE	LYS	ALA	ALA	PHE	ASP	MET	PHE	ASP
4	ALA	ASP	GLY	GLY	GLY	ASP	ILE	SER	THR	LYS
5	GLU	LEU	GLY	THR	VAL	MET	ARG	MET	LEU	GLY
6	GLN	ASN	PRO	THR	LYS	GLU	GLU	LEU	ASP	ALA
7	ILE	ILE	GLU	GLU	VAL	ASP	GLU	ASP	GLY	SER
8	GLY	THR	ILE	ASP	PHE	GLU	GLU	PHE	LEU	VAL
9	MET	MET	VAL	ARG	GLN	MET	LYS	GLU	ASP	ALA