BMRB Entry 19392

Name: Human Bcl10 CARD
Published: 2013-10-14

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PDB ID: 2mb9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19392
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human Bcl10 CARD

Deposition date:

2013-07-26

Original release date:

2013-10-14

Authors:

Zheng, Chao; Bracken, Clay; Wu, Hao

Citation:

Citation: Qiao, Qi; Yang, Chenghua; Zheng, Chao; Fontan, Lorena; David, Liron; Yu, Xiong; Bracken, Clay; Rosen, Monica; Melnick, Ari; Egelman, Edward; Wu, Hao. "Structural Architecture of the CARMA1/Bcl10/MALT1 Signalosome: Nucleation-Induced Filamentous Assembly" Mol. Cell 51, 766-779 (2013).
PubMed: 24074955

Assembly members:

Assembly members:
Bcl10, polymer, 118 residues, 12642.762 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Bcl10: GSHMEPTAPSLTEEDLTEVK KDALENLRVYLCEKIIAERH FDHLRAKKILSREDTREISC RTSSRKRAGKLLDYLQENPK GLDTLVESIRREKTQNFLIQ KITDEVLKLRNIKLEHLK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	466
15N chemical shifts	116
1H chemical shifts	777

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Bcl10_1	1
2	Bcl10_2	1
3	Bcl10_3	1
4	Bcl10_4	1
5	Bcl10_5	1
6	Bcl10_6	1
7	Bcl10_7	1
8	Bcl10_8	1
9	Bcl10_9	1
10	Bcl10_10	1
11	Bcl10_11	1
12	Bcl10_12	1
13	Bcl10_13	1
14	Bcl10_14	1
15	Bcl10_15	1
16	Bcl10_16	1
17	Bcl10_17	1
18	Bcl10_18	1
19	Bcl10_19	1
20	Bcl10_20	1

Entities:

Entity 1, Bcl10_1 118 residues - 12642.762 Da.

1

GLY

SER

HIS

MET

GLU

PRO

THR

ALA

PRO

SER

2

LEU

THR

GLU

ASP

LEU

THR

GLU

VAL

LYS

3

LYS

ASP

ALA

LEU

GLU

ASN

LEU

ARG

VAL

TYR

4

LEU

CYS

GLU

LYS

ILE

ALA

GLU

ARG

HIS

5

PHE

ASP

HIS

LEU

ARG

ALA

LYS

ILE

LEU

6

SER

ARG

GLU

ASP

THR

ARG

GLU

ILE

SER

CYS

7

ARG

THR

SER

ARG

LYS

ARG

ALA

GLY

LYS

8

LEU

ASP

TYR

LEU

GLN

GLU

ASN

PRO

LYS

9

GLY

LEU

ASP

THR

LEU

VAL

GLU

SER

ILE

ARG

10

ARG

GLU

LYS

THR

GLN

ASN

PHE

LEU

ILE

GLN

11

LYS

ILE

THR

ASP

GLU

VAL

LEU

LYS

LEU

ARG

12

ASN

ILE

LYS

LEU

GLU

HIS

LEU

LYS

Samples:

sample_1: Bcl10, [U-13C; U-15N], 0.3 – 0.4 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

Varian Unity 600 MHz

PDB	2MB9
DBJ	BAA88822 BAF84035 BAI45655
EMBL	CAA06955
GB	AAC99767 AAD15800 AAD16428 AAD24918 AAD32597
REF	NP_001071496 NP_001096683 NP_001248144 NP_003912 NP_112618
SP	O95999 Q9QYN5
TPG	DAA31335
AlphaFold	O95999 Q9QYN5