BMRB Entry 19052

Name: NMR Structure of the Complete Internal Fusion Loop mutant L529A/I544A from Ebolavirus GP2 at pH 5.5
Published: 2014-02-24

Click here to enlarge.

PDB ID: 2m5f
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19052
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR Structure of the Complete Internal Fusion Loop mutant L529A/I544A from Ebolavirus GP2 at pH 5.5

Deposition date:

2013-02-22

Original release date:

2014-02-24

Authors:

Gregory, Sonia; Tamm, Lukas

Citation:

Citation: Gregory, Sonia; Larsson, Per; Nelson, Elizabeth; Kasson, Peter; White, Judith; Tamm, Lukas. "Ebolavirus Entry Requires a Compact Hydrophobic Fist at the Tip of the Fusion Loop." J. Virol. ., .-. (2014).
PubMed: 24696482

Assembly members:

Assembly members:
Ebolavirus_Fusion_Loop_L529A/I544A, polymer, 54 residues, 5863.580 Da.

Natural source:

Natural source: Common Name: Enterobacteria Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ebolavirus_Fusion_Loop_L529A/I544A: AQPKCNPNLHYWTTQDEGAA IGAAWIPYFGPAAEGIYAEG LMHNQDGLICGLRQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	209
15N chemical shifts	49
1H chemical shifts	344

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ebolavirus_Fusion_Loop_L529A/I544A	1

Entities:

Entity 1, Ebolavirus_Fusion_Loop_L529A/I544A 54 residues - 5863.580 Da.

1

ALA

GLN

PRO

LYS

CYS

ASN

PRO

ASN

LEU

HIS

2

TYR

TRP

THR

GLN

ASP

GLU

GLY

ALA

3

ILE

GLY

ALA

TRP

ILE

PRO

TYR

PHE

GLY

4

PRO

ALA

GLU

GLY

ILE

TYR

ALA

GLU

GLY

5

LEU

MET

HIS

ASN

GLN

ASP

GLY

LEU

ILE

CYS

6

GLY

LEU

ARG

GLN

Samples:

sample_1: Ebolavirus Fusion Loop L529A/I544A, [U-15N], 600 uM; Phosphate buffer 30 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_2: Ebolavirus Fusion Loop L529A/I544A, [U-13C; U-15N], 600 uM; Phosphate buffer 30 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

Bruker Avance 600 MHz