BMRB Entry 18878

Name: Solution structure of the Hs. PSIP1 PWWP domain
Published: 2013-06-04

Click here to enlarge.

PDB ID: 3zeh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18878
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the Hs. PSIP1 PWWP domain

Deposition date:

2012-12-06

Original release date:

2013-06-04

Authors:

van Ingen, Hugo; Van nuland, R.; Timmers, H.; Boelens, R.

Citation:

Citation: van Nuland, Rick; van Schaik, Frederik Ma; Simonis, Marieke; van Heesch, Sebastiaan; Cuppen, Edwin; Boelens, Rolf; Timmers, Ht Marc; van Ingen, Hugo. "Nucleosomal DNA binding drives the recognition of H3K36-methylated nucleosomes by the PSIP1-PWWP domain." Epigenetics Chromatin 6, 12-12 (2013).
PubMed: 23656834

Assembly members:

Assembly members:
PC4_AND_SFRS1-INTERACTING_PROTEIN, polymer, 105 residues, 11890.6105 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: n/a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PC4_AND_SFRS1-INTERACTING_PROTEIN: GSHMAMARDFKPGDLIFAKM KGYPHWPARVDEVPDGAVKP PTNKLPIFFFGTHETAFLGP KDIFPYSENKEKYGKPNKRK GFNEGLWEIDNNPKVKFSSQ QAATK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	408
15N chemical shifts	90
1H chemical shifts	657

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PC4 AND SFRS1-INTERACTING PROTEIN	1

Entities:

Entity 1, PC4 AND SFRS1-INTERACTING PROTEIN 105 residues - 11890.6105 Da.

1

GLY

SER

HIS

MET

ALA

MET

ALA

ARG

ASP

PHE

2

LYS

PRO

GLY

ASP

LEU

ILE

PHE

ALA

LYS

MET

3

LYS

GLY

TYR

PRO

HIS

TRP

PRO

ALA

ARG

VAL

4

ASP

GLU

VAL

PRO

ASP

GLY

ALA

VAL

LYS

PRO

5

PRO

THR

ASN

LYS

LEU

PRO

ILE

PHE

6

GLY

THR

HIS

GLU

THR

ALA

PHE

LEU

GLY

PRO

7

LYS

ASP

ILE

PHE

PRO

TYR

SER

GLU

ASN

LYS

8

GLU

LYS

TYR

GLY

LYS

PRO

ASN

LYS

ARG

LYS

9

GLY

PHE

ASN

GLU

GLY

LEU

TRP

GLU

ILE

ASP

10

ASN

PRO

LYS

VAL

LYS

PHE

SER

GLN

11

GLN

ALA

THR

LYS

Samples:

sample_1: PC4 AND SFRS1-INTERACTING PROTEIN, [U-13C; U-15N], 0.5 mM; NaPi 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
triple resonance	sample_1	solution	sample_conditions_1
13C/15N edited NOESY	sample_1	solution	sample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

CNS vany, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - chemical shift assignment

CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

SPARKY vany, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 750 MHz

UNP	PSIP1_HUMAN
BMRB	18478
PDB	2M16 3ZEH 4FU6
DBJ	BAB27707 BAE24079 BAE36388 BAE41251 BAJ78791
EMBL	CAC34944 CAC34945
GB	AAC25167 AAC97945 AAC97946 AAF25870 AAF25871
REF	NP_001009372 NP_001075982 NP_001121689 NP_001137364 NP_001193405
SP	O75475 Q66T72 Q812D1 Q8MJG1 Q99JF8
TPG	DAA26946
AlphaFold	Q9UER6 O75475 Q66T72 Q812D1 Q8MJG1 Q99JF8