BMRB Entry 17702

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17702
MolProbity Validation Chart

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Title:
The protein complex for DNA replication
Deposition date:
2011-06-14
Original release date:
2012-12-17
Authors:
LIU, Changdong; WEI, Zhun; ZHU, Guang
Citation:

Citation: LIU, Changdong; WEI, Zhun; WU, Rentian; LIANG, Chun; ZHU, Guang. "The protein complex for DNA replication"  .

Assembly members:

Assembly members:
protein_comp1, polymer, 114 residues, 13304.011 Da.
protein_comp2, polymer, 29 residues, 3361.944 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
protein_comp1: APKASLRLGFSEYSRISNLI VLHLRKVEEEEDESALKRSE LVNWYLKEIESEIDSEEELI NKKRIIEKVIHRLTHYDHVL IELTQAGLKGSTEGSESYEE DPYLVVNPNYLLED
protein_comp2: SALKGVSQDLLERIRAKEAQ KQLAQMTRW

Data sets:
Data typeCount
13C chemical shifts363
15N chemical shifts125
1H chemical shifts771

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein_comp11
2protein_comp22

Entities:

Entity 1, protein_comp1 114 residues - 13304.011 Da.

1   ALAPROLYSALASERLEUARGLEUGLYPHE
2   SERGLUTYRSERARGILESERASNLEUILE
3   VALLEUHISLEUARGLYSVALGLUGLUGLU
4   GLUASPGLUSERALALEULYSARGSERGLU
5   LEUVALASNTRPTYRLEULYSGLUILEGLU
6   SERGLUILEASPSERGLUGLUGLULEUILE
7   ASNLYSLYSARGILEILEGLULYSVALILE
8   HISARGLEUTHRHISTYRASPHISVALLEU
9   ILEGLULEUTHRGLNALAGLYLEULYSGLY
10   SERTHRGLUGLYSERGLUSERTYRGLUGLU
11   ASPPROTYRLEUVALVALASNPROASNTYR
12   LEULEUGLUASP

Entity 2, protein_comp2 29 residues - 3361.944 Da.

1   SERALALEULYSGLYVALSERGLNASPLEU
2   LEUGLUARGILEARGALALYSGLUALAGLN
3   LYSGLNLEUALAGLNMETTHRARGTRP

Samples:

sample_1: MES 25 mM; sodium chloride 300 mM; glycerol 1%; protein_comp2, [U-100% 13C; U-100% 15N], 0.8 mM; protein_comp1 1 mM; H2O 90%; D2O 10%

sample_2: MES, [U-100% 15N], 25 mM; sodium chloride 300 mM; glycerol 1%; protein_comp1, [U-100% 13C; U-100% 15N], 0.8 mM; protein_comp2 1.0 mM; H2O 90%; D2O 10%

sample_3: MES 25 mM; sodium chloride 300 mM; glycerol 1%; protein_comp1, [U-100% 13C; U-100% 15N], 0.8 mM; protein_comp2 1.0 mM; D2O 100%

sample_4: MES 25 mM; sodium chloride 300 mM; glycerol 1%; protein_comp2, [U-100% 13C; U-100% 15N], 0.8 mM; protein_comp1 1.0 mM; D2O 100%

sample_conditions_1: ionic strength: 3 M; pH: 6.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
13C/15N filtered-C13 edited NOEsample_3isotropicsample_conditions_1
13C/15N filtered-C13 edited NOEsample_4isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_4isotropicsample_conditions_1

Software:

VNMR, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

HADDOCK1.3, Alexandre Bonvin - refinement

ProcheckNMR, Laskowski and MacArthur - geometry optimization

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 750 MHz

Related Database Links:

BMRB 16396 16796 16796
PDB
DBJ BAA12699 BAE01174 BAG35469 BAG61268
GB AAB48165 AAC50766 AAH32374 AAI12449 AAO26043 AAH14202 EAW66764 ELK00500
REF NP_001039699 NP_001247544 NP_001270984 NP_005906 XP_001154732 XP_006926820 XP_009249324 XP_009429704
SP Q14566 Q2KIZ8
AlphaFold Q14566 Q2KIZ8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks