BMRB Entry 17347

Name: Solution NMR structure of PAP248-286 in 30% TFE
Published: 2011-01-27

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PDB ID: 2l79
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17347
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of PAP248-286 in 30% TFE

Deposition date:

2010-12-03

Original release date:

2011-01-27

Authors:

NANGA, RAVI PRAKASH REDDY; BRENDER, JEFFREY; POPOVYCH, NATALIYA; RAMAMOORTHY, AYYALUSAMY

Citation:

Citation: Brender, Jeffrey; Nanga, Ravi Prakash Reddy; Popovych, Nataliya; Soong, Ronald; Macdonald, Peter; Ramamoorthy, Ayyalusamy. "The amyloidogenic SEVI precursor, PAP248-286, is highly unfolded in solution despite an underlying helical tendency." Biochim. Biophys. Acta 1808, 1161-1169 (2011).
PubMed: 21262195

Assembly members:

Assembly members:
entity, polymer, 39 residues, 4561.504 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GIHKQKEKSRLQGGVLVNEI LNHMKRATQIPSYKKLIMY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	269

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PAP248-286	1

Entities:

Entity 1, PAP248-286 39 residues - 4561.504 Da.

1

GLY

ILE

HIS

LYS

GLN

LYS

GLU

LYS

SER

ARG

2

LEU

GLN

GLY

VAL

LEU

VAL

ASN

GLU

ILE

3

LEU

ASN

HIS

MET

LYS

ARG

ALA

THR

GLN

ILE

4

PRO

SER

TYR

LYS

LEU

ILE

MET

TYR

Samples:

sample_1: PAP248-286 1.2 mM; sodium phosphate 20 mM; D2O, [U-99% 2H], 10%; TFE, [U-99% 2H], 30%

sample_conditions_1: ionic strength: 0 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

TALOS, Cornilescu, Delaglio and Bax - refinement

Molmol, Koradi, Billeter and Wuthrich - structure solution

SPARKY, Goddard - chemical shift assignment, data analysis

ProcheckNMR, Laskowski and MacArthur - data analysis, structure solution

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance 900 MHz

BMRB	17193 17346 17924 17925 18287
PDB	1CVI 1ND5 1ND6 2HPA 2L3H 2L77 2L79
DBJ	BAD89417 BAG62248
EMBL	CAA36422 CAA37673
GB	AAA60021 AAA60022 AAA69694 AAB60640 AAH07460
REF	NP_001090 NP_001127666 NP_001278966 XP_001115549 XP_001148736
SP	P15309
AlphaFold	P15309