BMRB Entry 17023

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17023
MolProbity Validation Chart

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Title:
Solution NMR structure of the ZNF216 A20 zinc finger
Deposition date:
2010-06-28
Original release date:
2012-08-02
Authors:
Garner, Thomas; Long, Jed; Searle, Mark; Layfield, Robert
Citation:

Citation: Garner, Thomas; Strachan, Joanna; Long, Jed; Layfield, Robert; Searle, Mark. "Co-localisation of ubiquitin receptors ZNF216 and p62 in a ubiquitin-mediated ternary complex"  Nat. Struct. Biol. ., .-..

Assembly members:

Assembly members:
ZNF216-A20, polymer, 62 residues, 6529.2 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PGEX-4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ZNF216-A20: GSMAQETNQTPGPMLCSTGC GFYGNPRTNGMCSVCYKEHL QRQQNSGRMSPMGTASGSNS PT

Data sets:
Data typeCount
13C chemical shifts207
15N chemical shifts65
1H chemical shifts276

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Znf_A201
2Zinc2

Entities:

Entity 1, Znf_A20 62 residues - 6529.2 Da.

Residues G1 amd S2 are non native artifacts from cloning

1   GLYSERMETALAGLNGLUTHRASNGLNTHR
2   PROGLYPROMETLEUCYSSERTHRGLYCYS
3   GLYPHETYRGLYASNPROARGTHRASNGLY
4   METCYSSERVALCYSTYRLYSGLUHISLEU
5   GLNARGGLNGLNASNSERGLYARGMETSER
6   PROMETGLYTHRALASERGLYSERASNSER
7   PROTHR

Entity 2, Zinc - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: ZNF216-A20 1 ± 0.1 mM; ZnCl2 50 ± 0.2 uM; DSS 0.1 ± 0.01 mM; TRIS 5 ± 0.5 mM; sodium chloride 50 ± 5 mM; H2O 90%; D2O 10%

sample_2: ZNF216-A20, [U-100% 15N], 1.2 ± 0.1 mM; ZnCl2 50 ± 0.2 uM; DSS 0.1 ± 0.01 mM; TRIS 5 ± 0.5 mM; sodium chloride 50 ± 5 mM; H2O 90%; D2O 10%

sample_3: ZNF216-A20, [U-100% 13C; U-100% 15N], 0.8 ± 0.2 mM; ZnCl2 50 ± 0.2 uM; DSS 0.1 ± 0.01 mM; TRIS 5 ± 0.5 mM; sodium chloride 50 ± 5 mM; H2O 90%; D2O 10%

sample_4: ZNF216-A20, [U-100% 15N], 1.2 ± 0.1 mM; ZnCl2 50 ± 0.2 uM; DSS 0.1 ± 0.01 mM; TRIS 5 ± 0.5 mM; sodium chloride 50 ± 5 mM; Polyacrylamide gel 7 ± 0.7 %; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HN(CA)COsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
2D 1H-15N HSQC IPAPsample_4anisotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CcpNMR v1.1.15, CCPN - chemical shift assignment, chemical shift calculation, data analysis, peak picking

X-PLOR NIH v2.14, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17024
PDB
DBJ BAC36321 BAF83538 BAG73932
GB AAC42600 AAC42601 AAC42602 AAC61801 AAH11018
REF NP_001090975 NP_001094515 NP_001095890 NP_001095891 NP_001157077
SP B5DF11 O76080 O88878
TPG DAA26877
AlphaFold B5DF11 O76080 O88878

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks