BMRB Entry 16561

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PDB ID: 2kpn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16561
MolProbity Validation Chart

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Title:
Solution NMR structure of a Bacterial Ig-like (Big_3) domain from Bacillus cereus. Northeast Structural Genomics Consortium target BcR147A.
Deposition date:
2009-10-16
Original release date:
2010-09-17
Authors:
Aramini, James; Wang, Dongyan; Ciccosanti, Colleen; Janjua, Haleema; Rost, Burkhard; Acton, Thomas; Xiao, Rong; Swapna, Gurla; Everett, John; Montelione, Gaetano
Citation:

Citation: Aramini, James; Wang, Dongyan; Ciccosanti, Colleen; Janjua, Haleema; Rost, Burkhard; Acton, Thomas; Xiao, Rong; Swapna, Gurla; Everett, John; Montelione, Gaetano. "Solution NMR structure of a Bacterial Ig-like (Big_3) domain from Bacillus cereus. Northeast Structural Genomics Consortium target BcR147A."  .

Assembly members:

Assembly members:
BcR147A, polymer, 103 residues, 11244.687 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Bacillus cereus   Taxonomy ID: 1396   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus cereus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BcR147A-658-751-21.1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BcR147A: MGNGETSDLEPKLTVPVGAT IHVGDSFVPMAEVLAIDKED GDLTSKIKVDGEVDTTKAGT YVLTYTVTDSKGHEVTAKQT VTVKVREEVKNDKPILEHHH HHH

Data typeCount
13C chemical shifts422
15N chemical shifts94
1H chemical shifts671

Time Domain Data

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1BcR147A1
2CALCIUM ION2

Entities:

Entity 1, BcR147A 103 residues - 11244.687 Da.

contains additional N-terminal Met and C-terminal LEHHHHHH affinity tag due to cloning.

1   METGLYASNGLYGLUTHRSERASPLEUGLU
2   PROLYSLEUTHRVALPROVALGLYALATHR
3   ILEHISVALGLYASPSERPHEVALPROMET
4   ALAGLUVALLEUALAILEASPLYSGLUASP
5   GLYASPLEUTHRSERLYSILELYSVALASP
6   GLYGLUVALASPTHRTHRLYSALAGLYTHR
7   TYRVALLEUTHRTYRTHRVALTHRASPSER
8   LYSGLYHISGLUVALTHRALALYSGLNTHR
9   VALTHRVALLYSVALARGGLUGLUVALLYS
10   ASNASPLYSPROILELEUGLUHISHISHIS
11   HISHISHIS

Entity 2, CALCIUM ION - Ca - 40.078 Da.

1   CA

Related Database Links:

SWS Q81D73_BACCR
PDB 2KPN

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