BMRB Entry 16015

Name: Structure of protein complex
Published: 2009-02-16

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PDB ID: 2ka6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR16015
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of protein complex

Deposition date:

2008-10-30

Original release date:

2009-02-16

Authors:

Wojciak, Jonathan; Martinez-Yamout, Maria; Dyson, Helen; Wright, Peter

Citation:

Citation: Wojciak, Jonathan; Martinez-Yamout, Maria; Dyson, Helen; Wright, Peter. "Structural basis for recruitment of CBP/p300 coactivators by STAT1 and STAT2 transactivation" EMBO J. 28, 948-958 (2009).
PubMed: 19214187

Assembly members:

Assembly members:
TAZ2, polymer, 92 residues, Formula weight is not available
STAT1, polymer, 45 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TAZ2: SPQESRRLSIQRCIQSLVHA CQCRNANCSLPSCQKMKRVV QHTKGCKRKTNGGCPVCKQL IALCCYHAKHCQENKCPVPF CLNIKHKLRQQQ
STAT1: GSHMSEVHPSRLQTTDNLLP MSPEEFDEVSRIVGSVEFDS MMNTV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	434
15N chemical shifts	142
1H chemical shifts	933

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TAZ2	1
2	STAT1	2
3	ZINC ION_1	3
4	ZINC ION_2	3
5	ZINC ION_3	3

Entities:

Entity 1, TAZ2 92 residues - Formula weight is not available

1

SER

PRO

GLN

GLU

SER

ARG

LEU

SER

ILE

2

GLN

ARG

CYS

ILE

GLN

SER

LEU

VAL

HIS

ALA

3

CYS

GLN

CYS

ARG

ASN

ALA

ASN

CYS

SER

LEU

4

PRO

SER

CYS

GLN

LYS

MET

LYS

ARG

VAL

5

GLN

HIS

THR

LYS

GLY

CYS

LYS

ARG

LYS

THR

6

ASN

GLY

CYS

PRO

VAL

CYS

LYS

GLN

LEU

7

ILE

ALA

LEU

CYS

TYR

HIS

ALA

LYS

HIS

8

CYS

GLN

GLU

ASN

LYS

CYS

PRO

VAL

PRO

PHE

9

CYS

LEU

ASN

ILE

LYS

HIS

LYS

LEU

ARG

GLN

10

GLN

Entity 2, STAT1 45 residues - Formula weight is not available

1

GLY

SER

HIS

MET

SER

GLU

VAL

HIS

PRO

SER

2

ARG

LEU

GLN

THR

ASP

ASN

LEU

PRO

3

MET

SER

PRO

GLU

PHE

ASP

GLU

VAL

SER

4

ARG

ILE

VAL

GLY

SER

VAL

GLU

PHE

ASP

SER

5

MET

ASN

THR

VAL

Entity 3, ZINC ION_1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: TAZ2, [U-100% 15N], 1 mM; STAT1; ZINC_ION; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 290 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

Bruker DRX 600 MHz
Bruker Avance 900 MHz

BMRB	16318
PDB	1F81 2KA6 2KJE
DBJ	BAE06125 BAI45616
EMBL	CAF96470 CAG06082 CDQ92618
GB	AAB28651 AAC17736 AAC51331 AAC51340 AAC51770
PRF	1923401A
REF	NP_001020603 NP_001073315 NP_001088637 NP_001157494 NP_001192159
SP	P45481 Q6JHU9 Q92793
TPG	DAA15549
AlphaFold	P45481 Q6JHU9 Q92793 Q9UDL5