BMRB Entry 11156

Name: Solution structure of the SH3 domain of human Nostrin
Published: 2011-05-05

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PDB ID: 2yun
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11156
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the SH3 domain of human Nostrin

Deposition date:

2010-04-15

Original release date:

2011-05-05

Authors:

Abe, H.; Tochio, N.; Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Abe, H.; Miyamoto, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SH3 domain of human Nostrin" .

Assembly members:

Assembly members:
SH3 domain, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050620-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGRLCKALYSFQARQ DDELNLEKGDIVIIHEKKEE GWWFGSLNGKKGHFPAAYVE ELPSNAGNTATKASGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	318
15N chemical shifts	73
1H chemical shifts	488

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3 domain	1

Entities:

Entity 1, SH3 domain 79 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

LEU

CYS

2

LYS

ALA

LEU

TYR

SER

PHE

GLN

ALA

ARG

GLN

3

ASP

GLU

LEU

ASN

LEU

GLU

LYS

GLY

ASP

4

ILE

VAL

ILE

HIS

GLU

LYS

GLU

5

GLY

TRP

PHE

GLY

SER

LEU

ASN

GLY

LYS

6

LYS

GLY

HIS

PHE

PRO

ALA

TYR

VAL

GLU

7

GLU

LEU

PRO

SER

ASN

ALA

GLY

ASN

THR

ALA

8

THR

LYS

ALA

SER

GLY

PRO

SER

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2YUN
REF	XP_005573448 XP_007963454 XP_010352711 XP_011902294